Direct Reprogramming of Human Dermal Fibroblasts Into Endothelial Cells Using ER71/ETV2.

ABSTRACT:

Rationale

Direct conversion or reprogramming of human postnatal cells into endothelial cells (ECs), bypassing stem or progenitor cell status, is crucial for regenerative medicine, cell therapy, and pathophysiological investigation but has remained largely unexplored.

Objective

We sought to directly reprogram human postnatal dermal fibroblasts to ECs with vasculogenic and endothelial transcription factors and determine their vascularizing and therapeutic potential.

Methods and results

We utilized various combinations of 7 EC transcription factors to transduce human postnatal dermal fibroblasts and found that ER71/ETV2 (ETS variant 2) alone best induced endothelial features. KDR⁺ (kinase insert domain receptor) cells sorted at day 7 from ER71/ETV2-transduced

SUBMITTER: Lee S 

PROVIDER: S-EPMC5336520 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature