Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV).

ABSTRACT:

Backgroud

Encephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.

Methods

In this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.

Results

We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, s

SUBMITTER: Wei J 

PROVIDER: S-EPMC5336634 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature