Fatty acid synthase regulates estrogen receptor-α signaling in breast cancer cells.

ABSTRACT: Fatty acid synthase (FASN), the key enzyme for endogenous synthesis of fatty acids, is overexpressed and hyperactivated in a biologically aggressive subset of sex steroid-related tumors, including breast carcinomas. Using pharmacological and genetic approaches, we assessed the molecular relationship between FASN signaling and estrogen receptor alpha (ERα) signaling in breast cancer. The small compound C75, a synthetic slow-binding inhibitor of FASN activity, induced a dramatic augmentation of estradiol (E₂)-stimulated, ERα-driven transcription. FASN and ERα were both necessary for the synergistic activation of ERα transcriptional activity that occurred following co-exposure to C75 and E₂: first, knockdown of FASN expression using RNAi (RNA interference) drastically lo

SUBMITTER: Menendez JA 

PROVIDER: S-EPMC5337623 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature