MicroRNA Modulation during the In vitro Culture of Hematopoietic Stem Cells Prior to Transplantation.

ABSTRACT:

Background

Human umbilical cord blood (HUCB) is an acceptable and readily accessible source of stem cells. There is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated HUCB cells. The involvement of miRNAs in several biological processes has been shown. The aim of this study was to evaluate the possible changes in miRNA expression profiles in CD133⁺ hematopoietic cells after in vitro culture.

Methods

HUCBCD133⁺ hematopoietic stem cells were isolated by magnetic-activated cell sorting, and then the cells were counted using flow cytometry. The cells were divided into 2 groups. In the first group, RNA was extracted and the cells of the seco

SUBMITTER: Shahrabi S 

PROVIDER: S-EPMC5337764 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature