Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus).

ABSTRACT:

Objective

An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.

Methods

In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization.

Results

Bioinformatics analysis showed that <

SUBMITTER: Jin M 

PROVIDER: S-EPMC5337911 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature