High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array.

ABSTRACT: Intercellular communication via chemical signaling proceeds with both spatial and temporal components, but analytical tools, such as microfabricated electrodes, have been limited to just a few probes per cell. In this work, we use a nonphotobleaching fluorescent nanosensor array based on single-walled carbon nanotubes (SWCNTs) rendered selective to dopamine to study its release from PC12 neuroprogenitor cells at a resolution exceeding 20,000 sensors per cell. This allows the spatial and temporal dynamics of dopamine release, following K⁺ stimulation, to be measured at exceedingly high resolution. We observe localized, unlabeled release sites of dopamine spanning 100 ms to seconds that correlate with protrusions but not predominately the positive curvature associated with the tip

SUBMITTER: Kruss S 

PROVIDER: S-EPMC5338365 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature