Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling.

ABSTRACT: To elucidate the dynamic features of a biologically relevant large-scale reaction network, we constructed a computational model of minimal protein synthesis consisting of 241 components and 968 reactions that synthesize the Met-Gly-Gly (MGG) peptide based on an Escherichia coli-based reconstituted in vitro protein synthesis system. We performed a simulation using parameters collected primarily from the literature and found that the rate of MGG peptide synthesis becomes nearly constant in minutes, thus achieving a steady state similar to experimental observations. In addition, concentration changes to 70% of the components, including intermediates, reached a plateau in a few minutes. However, the concentration change of each component exhibits several temporal plateaus, or a quasi-st

SUBMITTER: Matsuura T 

PROVIDER: S-EPMC5338406 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature