Barcoded nanoparticles for high throughput in vivo discovery of targeted therapeutics.

ABSTRACT: Nucleic acid therapeutics are limited by inefficient delivery to target tissues and cells and by an incomplete understanding of how nanoparticle structure affects biodistribution to off-target organs. Although thousands of nanoparticle formulations have been designed to deliver nucleic acids, most nanoparticles have been tested in cell culture contexts that do not recapitulate systemic in vivo delivery. To increase the number of nanoparticles that could be tested in vivo, we developed a method to simultaneously measure the biodistribution of many chemically distinct nanoparticles. We formulated nanoparticles to carry specific nucleic acid barcodes, administered the pool of particles, and quantified particle biodistribution by deep sequencing the barcodes. This method distinguished previous

SUBMITTER: Dahlman JE 

PROVIDER: S-EPMC5338412 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature