Kinetics, subcellular localization, and contribution to parasite virulence of a Trypanosoma cruzi hybrid type A heme peroxidase (TcAPx-CcP).

ABSTRACT: The Trypanosoma cruzi ascorbate peroxidase is, by sequence analysis, a hybrid type A member of class I heme peroxidases [TcAPx-cytochrome c peroxidase (CcP)], suggesting both ascorbate (Asc) and cytochrome c (Cc) peroxidase activity. Here, we show that the enzyme reacts fast with H₂O₂ (k = 2.9 × 10⁷ M^-1⋅s^-1) and catalytically decomposes H₂O₂ using Cc as the reducing substrate with higher efficiency than Asc (k_cat/K_m = 2.1 × 10⁵ versus 3.5 × 10⁴ M^-1⋅s^-1, respectively). Visible-absorption spectra of purified recombinant TcAPx-CcP after H₂O₂ reaction denote the formation of a

SUBMITTER: Hugo M 

PROVIDER: S-EPMC5338437 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature