Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser.

ABSTRACT: To understand how molecules function in biological systems, new methods are required to obtain atomic resolution structures from biological material under physiological conditions. Intense femtosecond-duration pulses from X-ray free-electron lasers (XFELs) can outrun most damage processes, vastly increasing the tolerable dose before the specimen is destroyed. This in turn allows structure determination from crystals much smaller and more radiation sensitive than previously considered possible, allowing data collection from room temperature structures and avoiding structural changes due to cooling. Regardless, high-resolution structures obtained from XFEL data mostly use crystals far larger than 1 μm³ in volume, whereas the X-ray beam is often attenuated to protect the detector f

SUBMITTER: Gati C 

PROVIDER: S-EPMC5338516 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature