Functional characterization in Caenorhabditis elegans of transmembrane worm-human orthologs.

ABSTRACT:

Background

The complete genome sequences for human and the nematode Caenorhabditis elegans offer an opportunity to learn more about human gene function through functional characterization of orthologs in the worm. Based on a previous genome-wide analysis of worm-human orthologous transmembrane proteins, we selected seventeen genes to explore experimentally in C. elegans. These genes were selected on the basis that they all have high confidence candidate human orthologs and that their function is unknown. We first analyzed their phylogeny, membrane topology and domain organization. Then gene functions were studied experimentally in the worm by using RNA interference and transcriptional gfp reporter gene fusions.

Results

The experiments gave functional insights for twelve of t

SUBMITTER: Henricson A 

PROVIDER: S-EPMC533873 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature