Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages.

ABSTRACT: Macrophages exhibit a phenotypic plasticity that enables them to orchestrate specific immune responses to distinct threats. The microbial product lipopolysaccharide (LPS) and the extracellular matrix glycoprotein tenascin-C are released during bacterial infection and tissue injury, respectively, and both activate Toll-like receptor 4 (TLR4). We found that these two TLR4 ligands stimulated distinct signaling pathways in macrophages, resulting in cells with divergent phenotypes. Although macrophages activated by LPS or tenascin-C displayed some common features, including activation of nuclear factor κB and mitogen-activated protein kinase signaling and cytokine synthesis, each ligand stimulated the production of different subsets of cytokines and generated different phosphoproteomic signatur

SUBMITTER: Piccinini AM 

PROVIDER: S-EPMC5338747 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature