Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature.

ABSTRACT:

Aim of the study

To assess the feasibility of partial HPV genotyping and methylation analysis of CADM1, MAL, and miR124-2 genes as triage tests in assaying self-collected cervical samples positive for high-risk HPV on primary screening, and to review the literature regarding host cellular gene methylation analysis of self-collected cervical samples.

Material and methods

Women residing in North-East Italy who had failed to respond to the invitation to participate in an organized population-based program were invited to provide a self-sample. Their stored baseline (self-collected) and follow-up (clinician-collected) cervical samples were included in the study. DNA was extracted from HPV-positive (Qiagen's Hybrid Capture 2, HC2) samples. Partial genotyping with separate detecti

SUBMITTER: Del Mistro A 

PROVIDER: S-EPMC5338782 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature