Sequencing the extrachromosomal circular mobilome reveals retrotransposon activity in plants.

ABSTRACT: Retrotransposons are mobile genetic elements abundant in plant and animal genomes. While efficiently silenced by the epigenetic machinery, they can be reactivated upon stress or during development. Their level of transcription not reflecting their transposition ability, it is thus difficult to evaluate their contribution to the active mobilome. Here we applied a simple methodology based on the high throughput sequencing of extrachromosomal circular DNA (eccDNA) forms of active retrotransposons to characterize the repertoire of mobile retrotransposons in plants. This method successfully identified known active retrotransposons in both Arabidopsis and rice material where the epigenome is destabilized. When applying mobilome-seq to developmental stages in wild type rice, we identified PopRice

SUBMITTER: Lanciano S 

PROVIDER: S-EPMC5338827 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature