The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi.

ABSTRACT: Filamentous fungi provide excellent systems for investigating the role of the AP-2 complex in polar growth. Using Aspergillus nidulans, we show that AP-2 has a clathrin-independent essential role in polarity maintenance and growth. This is in line with a sequence analysis showing that the AP-2 β subunit (β2) of higher fungi lacks a clathrin-binding domain, and experiments showing that AP-2 does not co-localize with clathrin. We provide genetic and cellular evidence that AP-2 interacts with endocytic markers SlaB^End4 and SagA^End3 and the lipid flippases DnfA and DnfB in the sub-apical collar region of hyphae. The role of AP-2 in the maintenance of proper apical membrane lipid and cell wall composition is further supported by its functional interaction with BasA

SUBMITTER: Martzoukou O 

PROVIDER: S-EPMC5338921 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature