Estrone Sulfate Transport and Steroid Sulfatase Activity in Colorectal Cancer: Implications for Hormone Replacement Therapy.

ABSTRACT: Hormone replacement therapy (HRT) affects the incidence and potential progression of colorectal cancer (CRC). As HRT primarily consists of estrone sulfate (E₁S), understanding whether this conjugated estrogen is transported and metabolized in CRC will define its potential effect in this malignancy. Here, we show that a panel of CRC cell lines (Colo205, Caco2, HCT116, HT-29) have steroid sulfatase (STS) activity, and thus can hydrolyze E₁S. STS activity is significantly higher in CRC cell lysate, suggesting the importance of E₁S transport in intracellular STS substrate availability. As E₁S transport is regulated by the expression pattern of certain solute carrier organic anion transporter polypeptides, we show that in CRC OATP4A1 is the most abund

SUBMITTER: Gilligan LC 

PROVIDER: S-EPMC5339229 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature