Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes.

ABSTRACT: Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T₀ generation were from 26.7 to 40%. Mutations in the homozygous T₀ lines stably transmitted to the T₁ generation and those in the bi-allelic lines segregated i

SUBMITTER: Sun Y 

PROVIDER: S-EPMC5339335 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature