Large-scale human skin lipidomics by quantitative, high-throughput shotgun mass spectrometry.

ABSTRACT: The lipid composition of human skin is essential for its function; however the simultaneous quantification of a wide range of stratum corneum (SC) and sebaceous lipids is not trivial. We developed and validated a quantitative high-throughput shotgun mass spectrometry-based platform for lipid analysis of tape-stripped SC skin samples. It features coverage of 16 lipid classes; total quantification to the level of individual lipid molecules; high reproducibility and high-throughput capabilities. With this method we conducted a large lipidomic survey of 268 human SC samples, where we investigated the relationship between sampling depth and lipid composition, lipidome variability in samples from 14 different sampling sites on the human body and finally, we assessed the impact of age and sex on

SUBMITTER: Sadowski T 

PROVIDER: S-EPMC5339821 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature