Lipid-Mediated Regulation of Embedded Receptor Kinases via Parallel Allosteric Relays.

ABSTRACT: Membrane-anchored receptors are essential cellular signaling elements for stimulus sensing, propagation, and transmission inside cells. However, the contributions of lipid interactions to the function and dynamics of embedded receptor kinases have not been described in detail. In this study, we used amide hydrogen/deuterium exchange mass spectrometry, a sensitive biophysical approach, to probe the dynamics of a membrane-embedded receptor kinase, EnvZ, together with functional assays to describe the role of lipids in receptor kinase function. Our results reveal that lipids play an important role in regulating receptor function through interactions with transmembrane segments, as well as through peripheral interactions with nonembedded domains. Specifically, the lipid membrane allosterically

SUBMITTER: Ghosh M 

PROVIDER: S-EPMC5340122 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature