A novel fission-independent role of dynamin-related protein 1 in cardiac mitochondrial respiration.

ABSTRACT:

Aims

Mitochondria in adult cardiomyocytes exhibit static morphology and infrequent dynamic changes, despite the high abundance of fission and fusion regulatory proteins in the heart. Previous reports have indicated that fusion proteins may bear functions beyond morphology regulation. Here, we investigated the role of fission protein, dynamin-related protein 1 (DRP1), on mitochondrial respiration regulation in adult cardiomyocytes.

Methods and results

By using genetic or pharmacological approaches, we manipulated the activity or protein level of fission and fusion proteins and found they mildly influenced mitochondrial morphology in adult rodent cardiomyocytes, which is in contrast to their significant effect in H9C2 cardiac myoblasts. Intriguingly, inhibiting endogenous DRP1

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5340145 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature