Whole-genome analysis of mycobacteria from birds at the San Diego Zoo.

ABSTRACT:

Methods

Mycobacteria isolated from more than 100 birds diagnosed with avian mycobacteriosis at the San Diego Zoo and its Safari Park were cultured postmortem and had their whole genomes sequenced. Computational workflows were developed and applied to identify the mycobacterial species in each DNA sample, to find single-nucleotide polymorphisms (SNPs) between samples of the same species, to further differentiate SNPs between as many as three different genotypes within a single sample, and to identify which samples are closely clustered genomically.

Results

Nine species of mycobacteria were found in 123 samples from 105 birds. The most common species were Mycobacterium avium and Mycobacterium genavense, which were in 49 and 48 birds, respectively. Most birds contained only a s

SUBMITTER: Pfeiffer W 

PROVIDER: S-EPMC5340394 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature