Immune reactivity after adenoviral-mediated aquaporin-1 cDNA transfer to human parotid glands.

ABSTRACT:

Objectives

The purpose of this study was to examine the humoral and cellular immune reactivity to adenoviral vector (AdhAQP1) administration in the human parotid gland over the first 42 days of a clinical gene therapy trial.

Methods

Of eleven treated subjects, five were considered as positive responders (Baum et al, 2012). Herein, we measured serum neutralizing antibody titers, circulating cytotoxic lymphocytes, and lymphocyte proliferation in peripheral blood mononuclear cells. Additionally, after adenoviral vector stimulation of lymphocyte proliferation, we quantified secreted cytokine levels.

Results

Responders showed little to modest immune reactivity during the first 42 days following gene transfer. Additionally, baseline serum neutralizing antibody titers to ser

SUBMITTER: Alevizos I 

PROVIDER: S-EPMC5340570 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature