CD31, a Valuable Marker to Identify Early and Late Stages of T Cell Differentiation in the Human Thymus.

ABSTRACT: Although CD31 expression on human thymocytes has been reported, a detailed analysis of CD31 expression at various stages of T cell development in the human thymus is missing. In this study, we provide a global picture of the evolution of CD31 expression from the CD34⁺ hematopoietic precursor to the CD45RA⁺ mature CD4⁺ and CD8⁺ single-positive (SP) T cells. Using nine-color flow cytometry, we show that CD31 is highly expressed on CD34⁺ progenitors and stays high until the early double-positive stage (CD3^-CD4⁺CD8α⁺β^-). After β-selection, CD31 expression levels become low to undetectable. CD31 expression then increases and peaks on CD3^highCD4⁺CD8⁺ double-positive

SUBMITTER: Douaisi M 

PROVIDER: S-EPMC5340597 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature