Proton pump inhibitor induced collagen expression in colonocytes is associated with collagenous colitis.

ABSTRACT:

Aim

To elucidate the role of proton pump inhibitors (PPIs) in collagenous disease, direct effect of PPI on colonocytes was examined.

Methods

Collagenous colitis is a common cause of non-bloody, watery diarrhea. Recently, there has been increasing focus on the use of proton PPIs as a risk factor for developing collagenous colitis. Mouse CT26 colonic cells were treated with PPI and/or PPI-induced alkaline media. Expression of fibrosis-associated genes was examined by RT-PCR. In human materials, collagen expression was examined by immunohistochemistry.

Results

CT26 cells expressed a Na⁺-H⁺ exchanger gene (solute carrier family 9, member A2). Treatment with PPI and/or PPI-induced alkaline media caused growth inhibition and oxidative stress in CT26 ce

SUBMITTER: Mori S 

PROVIDER: S-EPMC5340810 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature