Osmium tetroxide as a probe of RNA structure.

ABSTRACT: Structured RNAs have a central role in cellular function. The capability of structured RNAs to adopt fixed architectural structures or undergo dynamic conformational changes contributes to their diverse role in the regulation of gene expression. Although numerous biophysical and biochemical tools have been developed to study structured RNAs, there is a continuing need for the development of new methods for the investigation of RNA structures, especially methods that allow RNA structure to be studied in solution close to its native cellular conditions. Here we use osmium tetroxide (OsO₄) as a chemical probe of RNA structure. In this method, we have used fluorescence-based sequencing technologies to detect OsO₄ modified RNA. We characterized the requirements for OsO

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5340912 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature