A Luciferase-fragment Complementation Assay to Detect Lipid Droplet-associated Protein-Protein Interactions.

ABSTRACT: A critical challenge for all organisms is to carefully control the amount of lipids they store. An important node for this regulation is the protein coat present at the surface of lipid droplets (LDs), the intracellular organelles dedicated to lipid storage. Only limited aspects of this regulation are understood so far. For the probably best characterized case, the regulation of lipolysis in mammals, some of the major protein players have been identified, and it has been established that this process crucially depends on an orchestrated set of protein-protein interactions. Proteomic analysis has revealed that LDs are associated with dozens, if not hundreds, of different proteins, most of them poorly characterized, with even fewer data regarding which of them might physically interact. To c

SUBMITTER: Kolkhof P 

PROVIDER: S-EPMC5340998 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature