Novel methicillin resistance gene mecD in clinical Macrococcus caseolyticus strains from bovine and canine sources.

ABSTRACT: Methicillin-resistant Macrococcus caseolyticus strains from bovine and canine origins were found to carry a novel mecD gene conferring resistance to all classes of β-lactams including anti-MRSA cephalosporins. Association of β-lactam resistance with mecD was demonstrated by gene expression in S. aureus and deletion of the mecD-containing island in M. caseolyticus. The mecD gene was located either on an 18,134-bp M. caseolyticus resistance island (McRI_mecD-1) or a 16,188-bp McRI_mecD-2. Both islands were integrated at the 3' end of the rpsI gene, carried the mecD operon (mecD-mecR1_m-mecI_m), and genes for an integrase of the tyrosine recombinase family and a putative virulence-associated protein (virE). Apart from the mecD operon, that shared 66% ov

SUBMITTER: Schwendener S 

PROVIDER: S-EPMC5341023 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature