Size matters for in vitro gene delivery: investigating the relationships among complexation protocol, transfection medium, size and sedimentation.

ABSTRACT: Although branched and linear polyethylenimines (bPEIs and lPEIs) are gold standard transfectants, a systematic analysis of the effects of the preparation protocol of polyplexes and the composition of the transfection medium on their physicochemical behaviour and effectiveness in vitro have been much neglected, undermining in some way the identification of precise structure-function relationships. This work aimed to address these issues. bPEI/DNA and lPEI/DNA, prepared using two different modes of addition of reagents, gave rise to polyplexes with exactly the same chemical composition but differing in dimensions. Upon dilution in serum-free medium, the size of any kind of polyplex promptly rose over time while remained invariably stable in complete DMEM. Of note, the bigger the dimension of

SUBMITTER: Pezzoli D 

PROVIDER: S-EPMC5341125 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature