Identification and analysis of the stigma and embryo sac-preferential/specific genes in rice pistils.

ABSTRACT:

Background

In rice, the pistil is the female reproductive organ, and it consists of two stigmas and an ovary. The stigma is capable of receiving pollen grains and guiding pollen tube growth. The ovary holds the embryo sac, which is fertilized with male gametes to produce seed. However, little is known about the gene function and regulatory networks during these processes in rice.

Results

Here, using the RNA-Seq technique, we identified 3531 stigma-preferential genes and 703 stigma-specific genes within the rice pistils, and we verified 13 stigma-specific genes via qRT-PCR and in situ hybridization. The GO analysis showed that the transport-, localization-, membrane-, communication-, and pollination-related genes were significantly enriched in the stigma. Additionally, to ide

SUBMITTER: Yu L 

PROVIDER: S-EPMC5341191 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature