Prostaglandin E2 inhibits neutrophil extracellular trap formation through production of cyclic AMP.

ABSTRACT:

Background and purpose

Upon stimulation, neutrophils release their nuclear contents called neutrophil extracellular traps (NETs), which contain unfolded chromatin and lysosomal enzymes. NETs have been demonstrated to play a critical role in host defence, although the role of PGE2 , a bioactive substance generated in inflammatory tissues, in the formation of NETs remains unclear.

Experimental approach

The effects of PGE2 , agonists and antagonists of its receptors, and modulators of the cAMP-PKA pathway on the formation of NETs were examined in vitro in isolated neutrophils and in vivo in a newly established mouse model.

Key results

PGE2 inhibited PMA-induced NET formation in vitro through EP2 and EP4 Gαs-coupled receptors. Incubation with a cell-permeable cAMP analogu

SUBMITTER: Shishikura K 

PROVIDER: S-EPMC5341226 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature