Induction of endothelium-dependent constriction of mesenteric arteries in endotoxemic hypotensive shock.

ABSTRACT:

Background and purpose

Effective management of hypotension refractory to vasoconstrictors in severe sepsis is limited. A new strategy to ameliorate endotoxemic hypotension by inducing endothelium-dependent constriction of large arteries was assessed.

Experimental approach

Endotoxemia in rats was induced by injection of LPS (10 mg·kg(-1), i.v.). Haemodynamics were measured in vivo, reactivity of isolated mesenteric arteries by myography and expression of proteins and enzyme activities by immunohistochemistry, biochemistry and molecular biology.

Key results

Six hours after LPS, the hypotension was promptly reversed following injection (i.v. or i.p.) of oroxylin-A (OroA) . In isolated LPS-treated but not normal mesenteric arteries, OroA (1-10 μM) induced endothelium-depe

SUBMITTER: Tseng TL 

PROVIDER: S-EPMC5341340 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature