Self-Assembly and Anti-Amyloid Cytotoxicity Activity of Amyloid beta Peptide Derivatives.

ABSTRACT: The self-assembly of two derivatives of KLVFF, a fragment Aβ(16-20) of the amyloid beta (Aβ) peptide, is investigated and recovery of viability of neuroblastoma cells exposed to Aβ (1-42) is observed at sub-stoichiometric peptide concentrations. Fluorescence assays show that NH₂-KLVFF-CONH₂ undergoes hydrophobic collapse and amyloid formation at the same critical aggregation concentration (cac). In contrast, NH₂-K(Boc)LVFF-CONH₂ undergoes hydrophobic collapse at a low concentration, followed by amyloid formation at a higher cac. These findings are supported by the β-sheet features observed by FTIR. Electrospray ionization mass spectrometry indicates that NH₂-K(Boc)LVFF-CONH₂ forms a significant population of oligomeric speci

SUBMITTER: Castelletto V 

PROVIDER: S-EPMC5341572 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature