Genetic mosaics and time-lapse imaging identify functions of histone H3.3 residues in mouse oocytes and embryos.

ABSTRACT: During development from oocyte to embryo, genetic programs in mouse germ cells are reshaped by chromatin remodeling to orchestrate the onset of development. Epigenetic modifications of specific amino acid residues of core histones and their isoforms can dramatically alter activation and suppression of gene expression. H3.3 is a histone H3 variant that plays essential roles in mouse oocytes and early embryos, but the functional role of individual amino acid residues has been unclear because of technical hurdles. Here, we describe two strategies that successfully investigated the functions of three individual H3.3 residues in oogenesis, cleavage-stage embryogenesis and early development. We first generated genetic mosaic ovaries and blastocysts with stochastic expression of wild-type or muta

SUBMITTER: Zhou L 

PROVIDER: S-EPMC5341799 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature