The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila.

ABSTRACT: The Abl tyrosine kinase signaling network controls cell migration, epithelial organization, axon patterning and other aspects of development. Although individual components are known, the relationships among them remain unresolved. We now use FRET measurements of pathway activity, analysis of protein localization and genetic epistasis to dissect the structure of this network in Drosophila We find that the adaptor protein Disabled stimulates Abl kinase activity. Abl suppresses the actin-regulatory factor Enabled, and we find that Abl also acts through the GEF Trio to stimulate the signaling activity of Rac GTPase: Abl gates the activity of the spectrin repeats of Trio, allowing them to relieve intramolecular repression of Trio GEF activity by the Trio N-terminal domain. Finally, we show tha

SUBMITTER: Kannan R 

PROVIDER: S-EPMC5341800 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature