Assessing angiogenic responses induced by primary human prostate stromal cells in a three-dimensional fibrin matrix assay.

ABSTRACT: Accurate modeling of angiogenesis in vitro is essential for guiding the preclinical development of novel anti-angiogenic agents and treatment strategies. The formation of new blood vessels is a multifactorial and multi-stage process dependent upon paracrine factors produced by stromal cells in the local microenvironment. Mesenchymal stem cells (MSCs) are multipotent cells in adults that can be recruited to sites of inflammation and tissue damage where they aid in wound healing through regenerative, trophic, and immunomodulatory properties. Primary stromal cultures derived from human bone marrow, normal prostate, or prostate cancer tissue are highly enriched in MSCs and stromal progenitors. Using conditioned media from these primary cultures, a robust pro-angiogenic response was observed in

SUBMITTER: Brennen WN 

PROVIDER: S-EPMC5342079 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature