Fragile X mental retardation protein promotes astrocytoma proliferation via the MEK/ERK signaling pathway.

ABSTRACT:

Objective

To examine the association between fragile X mental retardation protein (FMRP) expression and astrocytoma characteristics.

Methods

Pathologic grade and expressions of glial fibrillary acidic protein (GFAP), Ki67 (proliferation marker), and FMRP were determined in astrocytoma specimens from 74 patients. Kaplan-Meier survival analysis was undertaken. Pathologic grade and protein levels of FMRP were determined in 24 additional patients with astrocytoma and 6 controls (cerebral trauma). In cultured U251 and U87 cell lines, the effects of FMRP knock-down on cell proliferation, AKT/mTOR/GSK-3β and MEK/ERK signaling were studied. The effects of FMRP knock-down on the volumes and weights of U251 cell-derived orthotopic tumors in mice were investigated.

Results

In pa

SUBMITTER: Xing Z 

PROVIDER: S-EPMC5342749 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature