Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.

ABSTRACT: Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OG_n) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1-2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n = 4+), and a stochastic mixture (n = 4_avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OG _n (n = 3, 4+, 4_avg) into RAW cells was significantly lower than G3-OG _n (n = 1, 2). The uptake

SUBMITTER: Vaidyanathan S 

PROVIDER: S-EPMC5342898 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature