A Non-invasive Method to Assess Hepatic Acetyl-CoA In Vivo.

ABSTRACT: Acetyl-coenzyme A (acetyl-CoA) is a critical metabolic signaling molecule that regulates gluconeogenesis, pyruvate oxidation, protein acetylation, and steroid and fatty acid biosynthesis; however, measurements of this metabolite using standard biochemical approaches are technically demanding, and there is currently no method to non-invasively assess hepatic acetyl-CoA content in vivo. To this end, we developed and validated a method to non-invasively detect differences in hepatic acetyl-CoA content in vivo across a 5-fold range of physiological acetyl-CoA concentrations by assessing the turnover of [¹³C₄]β-hydroxybutyrate (β-OHB). Here, we show a strong correlation (R² = 0.86, p < 0.0001) between hepatic acetyl-CoA content and β-OHB turnover in rats with va

SUBMITTER: Perry RJ 

PROVIDER: S-EPMC5342911 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature