Regulation of molecular clock oscillations and phagocytic activity via muscarinic Ca²⁺ signaling in human retinal pigment epithelial cells.

ABSTRACT: Vertebrate eyes are known to contain circadian clocks, however, the intracellular mechanisms regulating the retinal clockwork remain largely unknown. To address this, we generated a cell line (hRPE-YC) from human retinal pigmental epithelium, which stably co-expressed reporters for molecular clock oscillations (Bmal1-luciferase) and intracellular Ca²⁺ concentrations (YC3.6). The hRPE-YC cells demonstrated circadian rhythms in Bmal1 transcription. Also, these cells represented circadian rhythms in Ca²⁺-spiking frequencies, which were canceled by dominant-negative Bmal1 transfections. The muscarinic agonist carbachol, but not photic stimulation, phase-shifted Bmal1 transcriptional rhythms with a type-1 phase response curve. This is consistent with significant M3 muscari

SUBMITTER: Ikarashi R 

PROVIDER: S-EPMC5343479 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature