Decreased cytokine production by mononuclear cells after severe gram-negative infections: early clinical signs and association with final outcome.

ABSTRACT:

Background

Failure of circulating monocytes for adequate cytokine production is a trait of sepsis-induced immunosuppression; however, its duration and association with final outcome are poorly understood.

Methods

We conducted a substudy of a large randomised clinical trial. Peripheral blood mononuclear cells (PBMCs) were isolated within the first 24 h from the onset of systemic inflammatory response syndrome in 95 patients with microbiologically confirmed or clinically suspected gram-negative infections. Isolation was repeated on days 3, 7 and 10. PBMCs were stimulated for cytokine production. The study endpoints were the differences between survivors and non-survivors, the persistence of immunosuppression, and determination of admission clinical signs that can lead to early

SUBMITTER: Antonakos N 

PROVIDER: S-EPMC5343541 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature