Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification.

ABSTRACT: To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample-thus eliminating the need for additional sample manipulation, microchip redesigns, and/or system expansions required for traditional ISTD usage. Cs and Li ions were added as integrated ISTDs to the BGE, and their effects on the reproducibility of Na quantification were explored. Results were then compared to the conclusions of our previous publication which used Cs and Li as traditional ISTDs. The in-house fabricated microchips, electrophoretic protocols, and solu

SUBMITTER: Bidulock AC 

PROVIDER: S-EPMC5343550 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature