Cytochrome P450s in human immune cells regulate IL-22 and c-Kit via an AHR feedback loop.

ABSTRACT: The mechanisms how environmental compounds influence the human immune system are unknown. The environmentally sensitive transcription factor aryl hydrocarbon receptor (AHR) has immune-modulating functions and responds to small molecules. Cytochrome P4501 enzymes (CYP1) act downstream of the AHR and metabolize small molecules. However, it is currently unknown whether CYP1 activity is relevant for immune modulation. We studied the interdependence of CYP1 and AHR in human primary immune cells using pharmacological methods. CYP1 inhibition increased the expression levels of the stem cell factor receptor (c-Kit) and interleukin (IL)-22 but decreased IL-17. Single cell analyses showed that CYP1 inhibition especially promoted CD4⁺ helper T (Th) cells that co-express c-Kit and IL-22 sim

SUBMITTER: Effner R 

PROVIDER: S-EPMC5343665 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature