Mrpl10 and Tbp Are Suitable Reference Genes for Peripheral Nerve Crush Injury.

ABSTRACT: Peripheral nerve injury triggers the dysregulation of a large number of genes at multiple sites, including neurons, peripheral nerve stump, and the target organ. Housekeeping genes were frequently used as reference genes to normalize the expression values of target genes. Suitable selection of housekeeping genes that are stably expressed after nerve injury minimizes bias elicited by reference genes and thus helps to better and more sensitively reflect gene expression changes. However, many housekeeping genes have been used as reference genes without testing the expression patterns of themselves. In the current study, we calculated the expression stability of nine commonly used housekeeping genes, such as 18S (18S ribosomal RNA), Actb (β-actin), CypA (cyclophilin A),

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5343799 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature