Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.

ABSTRACT: Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.

SUBMITTER: Blixt O 

PROVIDER: S-EPMC534418 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature