MicroRNA promoter methylation: a new tool for accurate detection of urothelial carcinoma.

ABSTRACT:

Background

Urothelial carcinoma (UC) is the most common cancer affecting the urinary system, worldwide. Lack of accurate early detection tools entails delayed diagnosis, precluding more efficient and timely treatment. In a previous study, we found that miR-129-2 and miR-663a were differentially methylated in UC compared with other genitourinary tract malignancies. Here, we evaluated the diagnostic performance of those microRNAs in urine.

Methods

Promoter methylation levels of miR-129-2 and miR-663a were assessed, using real-time quantitative methylation-specific PCR, in UC tissue samples (using normal urothelium as control) and, subsequently, in urine samples from UC and other genitourinary malignancies. Diagnostic and prognostic performances were evaluated by receiver opera

SUBMITTER: Padrao NA 

PROVIDER: S-EPMC5344289 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature