Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.

ABSTRACT:

Background

Immunity to the sand fly salivary protein SALO (Salivary Anticomplement of Lutzomyia longipalpis) protected hamsters against Leishmania infantum and L. braziliensis infection and, more recently, a vaccine combination of a genetically modified Leishmania with SALO conferred strong protection against L. donovani infection. Because of the importance of SALO as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowledge of its structural characteristics are needed to move SALO forward for the clinical path.

Methodology/principal findings

Recombinant SALO was expressed as a soluble secreted protein using Pichia pastoris, rSALO(P), with yields of 1g/L and >99% purity as assessed by SEC-

SUBMITTER: Asojo OA 

PROVIDER: S-EPMC5344329 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature