The role of platelet and endothelial GARP in thrombosis and hemostasis.

ABSTRACT:

Background

Glycoprotein-A Repetitions Predominant protein (GARP or LRRC32) is present on among others human platelets and endothelial cells. Evidence for its involvement in thrombus formation was suggested by full knockout of GARP in zebrafish.

Objectives

To evaluate the role of GARP in platelet physiology and in thrombus formation using platelet and endothelial conditional GARP knock out mice.

Methods

Platelet and endothelial specific GARP knockout mice were generated using the Cre-loxP recombination system. The function of platelets without GARP was measured by flow cytometry, spreading analysis and aggregometry using PAR4-activating peptide and collagen related peptide. Additionally, clot retraction and collagen-induced platelet adhesion and aggregation under flow

SUBMITTER: Vermeersch E 

PROVIDER: S-EPMC5344406 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature