Robust growth of avirulent phase II Coxiella burnetii in bone marrow-derived murine macrophages.

ABSTRACT: Published data show that murine bone marrow-derived macrophages (BMDM) restrict growth of avirulent phase II, but not virulent phase I, Coxiella burnetii. Growth restriction of phase II bacteria is thought to result from potentiated recognition of pathogen-associated molecular patterns, which leads to production of inhibitory effector molecules. Past studies have used conditioned medium from L-929 murine fibroblasts as a source of macrophage-colony stimulating factor (M-CSF) to promote differentiation of bone marrow-derived myeloid precursors into macrophages. However, uncharacterized components of conditioned medium, such as variable amounts of type I interferons, can affect macrophage activation status and their permissiveness for infection. In the current study, we show that the C. burn

SUBMITTER: Cockrell DC 

PROVIDER: S-EPMC5344453 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature