Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn.

ABSTRACT: Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of th

SUBMITTER: Betekhtin A 

PROVIDER: S-EPMC5344457 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature