The human plasma-metabolome: Reference values in 800 French healthy volunteers; impact of cholesterol, gender and age.

ABSTRACT: Metabolomic approaches are increasingly used to identify new disease biomarkers, yet normal values of many plasma metabolites remain poorly defined. The aim of this study was to define the "normal" metabolome in healthy volunteers. We included 800 French volunteers aged between 18 and 86, equally distributed according to sex, free of any medication and considered healthy on the basis of their medical history, clinical examination and standard laboratory tests. We quantified 185 plasma metabolites, including amino acids, biogenic amines, acylcarnitines, phosphatidylcholines, sphingomyelins and hexose, using tandem mass spectrometry with the Biocrates AbsoluteIDQ p180 kit. Principal components analysis was applied to identify the main factors responsible for metabolome variability and orthog

SUBMITTER: Trabado S 

PROVIDER: S-EPMC5344496 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature